Microbial strain adaptation

Microbes show a surprisingly capability to adapt to changing environments, this is due to the possibility for a given genotype to express remarkably different phenotypes. Indeed, any given phenotype can be regarded as a fixed-point attractor in the phenotype space. When properly perturbed, microbes can “jump” from one attractor to another thus displaying different “attitudes” (i.e. phenotypes) toward experimental conditions such as temperature, pH, contaminants and solvent concentration.
This feature of microbes allows to optimize a given strain to specific experimental condition without the need for extensive genetic engineering significantly shortening experimental time. At Explora we have learnt how to trigger phenotype “jumps” so that we can induce strain adaptation to meet your process technical requirements.

You provide us:

• your target protein transformed into a strain of your choice
• process technical requirements you want the strain to be adapted to

We deliver you:

• Identification of critical process parameters
• One or more adapted strain as glycerol stock

*mandatory fields
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	STEP1. Construct Details
Construct Name *		e.g. pET22b(+)/GFP_V236L
Plasmid type:	low copy high copy
Antibiotic Resistance: *	e.g. ampicillin
Which promotor is cloned upstream your target gene?	e.g. T7-LacI
Which inducer controls your target gene expression?	e.g None (gene is constitutively expressed), IPTG, arabinose
Is you protein toxic to E.coli? *	No Yes I do not know
Is your protein a monomer? *	Yes it is a monomer No it is a dimer No it is a trimer No it is a multimer I do not know.
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	STEP2. Strain Details
Microbial strain? *	e.g. E.coli BL21 or B.subtilis WB800N
Has this strain already/ever been adapted? *	Yes No
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	STEP3. Your target conditions
Target conditions: *	e.g. Terrific Broth, pH 5.7, 30°C, NaCl 0.5M, Glycerol 1%, PMSF 0.1mM, PEG 3000 2%	Please specify experimental condition you want your strain to be adapted to.
	STEP4. Your details
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Full Name *
Your Institution *		Please provide us institution name, Country, Streeet, ZIP Code
Your Email
Your Phone		e.g 001-901-987654321
Enter code (dark letters)