Simplify you life with Explora's gene synthesis service. Design your custom gene to meet exactly your needs:

• Any sequence with no costrain with regard to GC
• Fully sequence verified
• Optimise protein expression by codon adaption
• Introduce several mutations
• Quickly add N- or C-terminal affinity tags.
• Insert linkers.
• Create fusion proteins or fusion transcripts.
• Choose from a broad range of promoter
• Add or remove sites for protein modifications such as phosphorylation, glycosylation or PEGylation.
• Add or remove restriction sites to facilitate downstream gene manipulations.

Don't waste your time on cloning, sequencing and purification - Order your gene online and concentrate on research!

Fill in the following form to ask for a quote.

*mandatory fields
_______________________________	_______________________________________	__________________________________
	STEP1. Design your construct
Choose you promotor	NoneT7 T3 SP6 SP3 T7/LacO promoter (inducible)pLac/LacO (inducible)TetR (repressible)Lambda pR promotor (repressible)SV40 promoterCMV promoter
Leader peptide	NonePelBOmpAgIIIOther	Leader peptide (LP) defines protein cellular localization. LP is usually removed cleaved off to yield the mature protein
N-terminus tag	NonecMycFLAG tagHis tagHalo tag
N-term protease cleavage site	NoneThrombineFactor Xa proteaseTEV proteaseTrypsinEnterokinase	The N-terminus protease cleavage site is used to cleave off the N-tag from the mature protein.
5'- restriction site	Explora's standard 5'-RE
Gene name *
Accession Number
Upload gene sequence *
Optimize for *	E.coli L.lactisP.pastoris P.methanolicaS.cerevisiaeBaculovirusInsect Cells (Stable recombinant cell lines)Insect Cells (Schneider cells-Drosophila)Rabbit reticulocyte lysateWheat germ extractE.coli extracts
Avoid the following restriction sites in my gene	e.g. NdeI, NcoI, BamHI
Additional feature	e.g. add/remove phosphorilation, glycosylation or PEGylation site
3'- restriction site	Explora's standard 3'-RE
C-term protease cleavage site	NoneThrombineFactor Xa proteaseTEV proteaseTrypsinEnterokinase	The C-terminus protease cleavage site is used to cleave off the C-tag and the carrier protein from the mature protein.
C-terminus tag	NonecMycFLAG tagHis tagHalo tag
Carrier Protein	NoneMaltose Binding Protein (MBP)Glutathione S-Trasferase (GST)Thioredoxin (TRX)Green Fluorescent Ptotein (GFP)M13 Minor Coat Protein (pIII)	Carrier protein may increase solubility or folding
	STEP2. Design your destination vector
Destination Vector *	Explora Standard Vector (pEX-0001) Other vector provided by the customer
Deliver as *	10ug purified plasmid (MiniPrep)100 ug purified plasmid (MiniPrep) 500 ug purified plasmid (MidiPrep) 1000 ug purified plasmid (MaxiPrep)
Purity *	Standard Endotoxin-free Transfection grade
Transform my gene construct into:		If you want us to transform your construct into a specific cell type, please type cell strain name, Manufacturer, Cat nr. Otherwise leave this field blank.
Deliver cells as	Stab culture Glycerol Stock	If you chose to have your construct transformed, please specify how we shall deliver the cells. Otherwise leave this field blank.
	STEP3. Your details
Full Name *
Your Institution *		Please provide us institution name, Country, Streeet, ZIP Code
Your Email
Your Phone		e.g 001-901-987654321
Enter code (dark letters)