Synthetic Gene Libraries

Whether you plan to investigate structure-activity relationship or modify proteins activity, synthetic gene libraries are the tool of choice. A library is designed and constructed to sample a specific set of sequence variations which are subsequently screened to identify variants with improved or novel properties. Explora provides six dynamic library options to enable you to focus your time directly on your research while quickly achieving significant results:

• Alanine scannining: replace one or more wild-type amino acid with alanine to investigate the contribution of an individual amino acid sidechain to the functionality of the protein

• Site-directed mutagenesis: specified mutations at one or more specific locations to rationally engineer protein activity such as Kcat, Km, allosteric features, substrate specificity, enhanced stability to pH, temperature and solvents

• Site-saturated Libraries: All 19 non wild-type substitutions at one or more specific locations to identify favorable amino acid substitutions at specified residues.

• Combinatorial Libraries: Simultaneous degeneration of multiple codons to achieve maximum diversity to identify proteins variants where epistatic and long-range effects play a major role.

• Truncation Libraries: Incremental truncation libraries generated by trimming 5' and 3' ends of a gene to identify core structures.

Do you need assistance to choose the best possible option for your research project? Contact our Library Design Specialists at This e-mail address is being protected from spambots. You need JavaScript enabled to view it