BIOPROCESS OPTIMIZATION

E. coli production process optimization and scale-up

Explora can optimize your protein expression improving simultaneously protein yield, solubility and cellular localization using its proprietary and patent-pending algorithm (have a look to our case study here). Process optimization involves the simultaneous optimization of several parameters (e.g. suitable carbon and energy sources, nitrogen sources, other key macro-nutrients, micro-nutrients, and the effects of inducers/repressors of enzyme production temperature and process variables such as pH, dissolved oxygen, Pressure, agitation and stirring effects) which defines high-dimensional experimental space that cannot be exhaustively explored experimentally. In addition, the simultaneous optimization of different parameters often results in trade-off between parameters’ optima and global optima. This set of trade-off designs is known as a Pareto set and requires appropriate statistical tools. To tackle the problem, we develped a aptent-pending algorithm to search experimental space for global optima.
Moreover, once the optimal conditions are identified we can scale-up the fermentation process up to 20L under non-GMP condition. In collaboration with our partners, the fermentation process can be scaled up to 2000 L production volume under GMP conditions.

You provide us:

  • your target protein cloned into a ready-to-express vector

We deliver you:

  • Identification of critical process parameters
  • Development of nutrient feed strategies
  • Detailed protocol for optimal expression of your protein
  • Pilot plant design
  • Scale-up design

QUOTE REQUEST FORM

Protein name*
Protein Accession Number
Upload sequence
Is your protein a monomer?
Has your protein an affinity tag?
Preffered Strain*
Preferred fermentation system?
Do you have already a fermentation equipment you want the bioprocess to be adapted to?

insert here the details of your fermentation system if you have any. E.g. Manufacturer Name, Size, On-line detector available, impeller type, ect ect

Production Target
Target purity
Shall your protein be insoluble, do you want us to attempt refolding?
Do you want us to develop an activity test for your recombinant product?

Your name*
Your email*